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| Yamagishi, J.; Watanabe, J.; Goo, Y. K.; Masatani, T.; Suzuki, Y.; Xuan, X.; 玄, 学南. |
| The 5′ UTR is widely involved in gene expression via post-transcriptional regulation. However, a detailed profile of the 5′ UTR for Toxoplasma gondii has not yet been demonstrated. To investigate the issue, we compared the predicted open reading frames (ORFs) and transcription start sites (TSSs) of T. gondii obtained by TSS-seq, a method that enables analysis of encyclopedic TSSs with next-generation sequencers. As a result, it was demonstrated that the mode length of the 5′ UTR is between 120 and 140 nucleotides (nts) when a subset of genes with predicted signal peptides was examined. However, when genes without the signal peptide were examined, the length was extended to approximately 600 nts. Because additional information on the predicted signal... |
| Ano: 2012 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3822 |
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| Kamyingkird, K.; Goo, Y.K.; Cao, S.; Adjou Moumouni, P.K.; Aboge, G.O.; Yamagishi, J.; Terkawi, M.A.; Masatani, T.; Yu, L.; Nishikawa, N.; Xuan, X.. |
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Palavras-chave: Aspartic protease; Toxoplasma gondii. |
| Ano: 2014 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4093 |
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| Kumagai, A.; Zhang, G.; Jia, H.; Nakamura, C.; Zhang, H.; Goo, Y-K.; Aboge, G. A.; Terkawi, M. A.; Zhou, J.; Nishikawa, Y.; Xuan, X.. |
| A gene encoding Babesia gibsoni thrombospondin-related adhesive protein (BgTRAP), known as a vaccine candidate, was stably expressed in Toxoplasma gondii (Tg/BgTRAP). The molecular weight and the antigenic reaction of recombinant BgTRAP expressed by the Tg/BgTRAP were similar to the original ones expressed by B. gibsoni. To evaluate the antigenicity of the recombinant BgTRAP expressed by T. gondii, the lysates of the recombinant parasite tachyzoites were intraperitoneally injected into mice. The serum collected from Tg/BgTRAP-immunized mouse could react to B. gibsoni parasites, while the serum collected from wild-type T. gondii tachyzoites (Tg/wt)-immunized mice did not. These results indicate that T. gondii could provide a new tool to produce foreign... |
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Palavras-chave: Toxoplasma gondii; Babesia gibsoni; BgTRAP; Expression; Vaccine. |
| Ano: 2016 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4496 |
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| Kamyingkird, K.; Yangtara, S.; Desquesnes, M.; Cao, S.; Adjou Moumouni, P.K.; Jittapalapong, S.; Nimsupan, S.; Terkawi, M.A.; Masatani, T.; Nishikawa, Y.; Igarashi, I.; Xuan, X.. |
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Palavras-chave: Babesia caballi; ELISA; IFAT; PCR; Theileria equi. |
| Ano: 2014 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4092 |
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